Cleavage by trypsin and by the proteinase from Armillaria mellea at ɛ-N-formyl-lysine residues
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چکیده
منابع مشابه
Trypsin-specific Inhibitors from the Macrolepiota procera, Armillaria mellea and Amanita phalloides wild mushrooms.
Wild growing mushrooms are a rich source of novel proteins with unique features. We have isolated and characterized trypsin inhibitors from two edible mushrooms, the honey fungus (Armillaria mellea) and the parasol mushroom (Macrolepiota procera), and from the poisonous death cap (Amanita phalloides). The trypsin inhibitors isolated: armespin, macrospin and amphaspin, have similar molecular mas...
متن کاملThe root rot fungus Armillaria mellea introduced into South Africa by early Dutch settlers.
Dead and dying oak (Quercus) and numerous other woody ornamental trees and shrubs showing signs and symptoms of Armillaria root rot were identified in the Company Gardens, Cape Town, South Africa, which were established in the mid-1600s by the Dutch East Indies Trading Company. Nineteen isolates from dying trees or from mushrooms were collected and analysed to identify and characterize the Armi...
متن کاملTrypsin cleaves exclusively C-terminal to arginine and lysine residues.
Almost all large-scale projects in mass spectrometry-based proteomics use trypsin to convert protein mixtures into more readily analyzable peptide populations. When searching peptide fragmentation spectra against sequence databases, potentially matching peptide sequences can be required to conform to tryptic specificity, namely, cleavage exclusively C-terminal to arginine or lysine. In many pub...
متن کاملMicrosatellite markers for the diploid basidiomycete fungus Armillaria mellea.
We isolated and characterized 12 microsatellite markers for two North American populations (California, Pennsylvania) of Armillaria mellea, a fungal pathogen responsible for Armillaria root disease of numerous woody plants. Allele frequency ranged from two to nine alleles per locus, and gene diversity ranged from 0.05 to 0.86. Of the 12 loci, eight loci were polymorphic in the California and Pe...
متن کاملArmillaria mellea: phenolic content, in vitro antioxidant and antihyperglycemic effects.
UNLABELLED Armillaria mellea, known as honey mushroom, has been used both as food and medicine. AIM In this work, the ethanolic and hydromethanolic extracts from Armillaria mellea fruiting bodies were investigated for their phenolic content, antioxidant and antihyperglycemic effects (in vitro studies). MATERIAL AND METHODS The total phenolics were quantified using Folin-Ciocalteu assay. The...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1981
ISSN: 0264-6021
DOI: 10.1042/bj1930737